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GliderGuider

(21,088 posts)
Wed Jun 11, 2014, 01:55 PM Jun 2014

Nucleic Acid Invaders from Food Confirmed

Just as many of us suspected all along.

Nucleic Acid Invaders from Food Confirmed

New research confirms that DNA fragments derived from meals, large enough to carry complete genes, can escape degradation and enter the human circulatory system, and so can RNA, raising serious concerns over new nucleic acids introduced into the human food chain via genetically modified organisms.

A study led by Sándor Spisák who holds a joint appointment at Hungarian Academy of Sciences in Budapest and Harvard Medical School Boston, Massachusetts in the USA analysed over 1,000 human adult samples from four independent studies, and found DNA fragments derived from food in all plasma samples, some large enough to code for complete genes.

Previous animal feeding studies have demonstrated that a minor proportion of fragmented dietary DNA may resist digestion, but the degradation of long chains of DNA and the possible uptake and transport into the bloodstream are not at all understood. Circulating cell free DNA (cfDNA) in the human bloodstream, first described in 1948, are mostly double-stranded molecules with a wide range of fragment sizes from 180 - 21 k bp.

Most people think cfDNA are from apoptotic cells (resulting from programmed cell-death), and in different diseases such as inflammation, autoimmune, trauma and cancer, necrotic cells (from non-programmed cell death) may increase the amount. In fact, both DNA and RNA are found circulating in the bloodstream, and there is good evidence that they are actively secreted from living cells as a nucleic acid intercommunication system.

Ruh-roh...
44 replies = new reply since forum marked as read
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Nucleic Acid Invaders from Food Confirmed (Original Post) GliderGuider Jun 2014 OP
You are what you eat superpatriotman Jun 2014 #1
My sister in law is in the industry....she says this is NOT unexpected and to not be alarmed VanillaRhapsody Jun 2014 #2
Scientists can be every bit as risk-blind as laymen. GliderGuider Jun 2014 #3
She has a new tiny baby....who she is only feeding adult food. ...not baby food. VanillaRhapsody Jun 2014 #4
People "in the industry" with PhDs miscalculate risks all the time. GliderGuider Jun 2014 #5
there is no way to do that....if you eat it...it enters your body.... VanillaRhapsody Jun 2014 #6
UMmm NOT with what they are genetically modifying with...IE PIG Genes Frog Genes ...give me a break. Drew Richards Jun 2014 #29
Should I instead trust MattBaggins Jun 2014 #10
"Random internet article" ... What a neutral characterization! GliderGuider Jun 2014 #17
That's what it is; a random internet article from a writer with a known agenda. REP Jun 2014 #20
Can you point me towards evidence to that effect? GliderGuider Jun 2014 #22
#19 and #21 REP Jun 2014 #24
Ah, you disagree with his opinion! OK then. GliderGuider Jun 2014 #33
Well yes. I know my ass from third base. Ho not so much. REP Jun 2014 #37
Random internet articles can contain............anything. Enthusiast Jun 2014 #26
Scientists themselves can be falliable, this is true. AverageJoe90 Jun 2014 #44
She's in the industry profiting from this. Enough said. n/t pnwmom Jun 2014 #8
Your precious Saint Mercola likes to collect a paycheck MattBaggins Jun 2014 #11
I have no idea who you're talking about. n/t pnwmom Jun 2014 #13
this appears to be coming from legitimate sourse G_j Jun 2014 #7
Not so much REP Jun 2014 #19
interesting G_j Jun 2014 #23
Science fact seem to be his thing REP Jun 2014 #25
Upon looking further G_j Jun 2014 #27
He seems especially dismissive of expensive water, or homeopathy as it is often called REP Jun 2014 #36
Your post demonstrates the need for better eductation jeff47 Jun 2014 #9
The conclusion of the report says this: GliderGuider Jun 2014 #12
Monsanto has to sell its GMO's so they can sell their herbicides pnwmom Jun 2014 #15
And that conclusion is talking about what we discovered in 1948. jeff47 Jun 2014 #18
The problem may not be my lack of education GliderGuider Jun 2014 #31
If it isn't, you're welcome to explain how the cfDNA gets expressed. (nt) jeff47 Jun 2014 #38
My degree is not in molecular biology. However, GliderGuider Jun 2014 #41
Not genes being expressed. jeff47 Jun 2014 #43
A couple of comments GliderGuider Jun 2014 #40
:eyes: jeff47 Jun 2014 #42
basically what my Sister in Law explained to me when I showed her this article a couple weeks back VanillaRhapsody Jun 2014 #16
Although it might look like it, that MineralMan Jun 2014 #14
About the Institute and Ho: REP Jun 2014 #21
It sounds to me like Ho has opinions you disagree with. GliderGuider Jun 2014 #30
Not confirmed! Hyperbole and mis-information. Non-scientific... yawnmaster Jun 2014 #28
K&R! DeSwiss Jun 2014 #32
Hmmm. Well would you look at that. GliderGuider Jun 2014 #34
Yeah..... DeSwiss Jun 2014 #35
Don't let George Bush know about this. L0oniX Jun 2014 #39
 

VanillaRhapsody

(21,115 posts)
2. My sister in law is in the industry....she says this is NOT unexpected and to not be alarmed
Wed Jun 11, 2014, 02:01 PM
Jun 2014

She has a PhD. And is a Scientist working on proteins...

 

VanillaRhapsody

(21,115 posts)
4. She has a new tiny baby....who she is only feeding adult food. ...not baby food.
Wed Jun 11, 2014, 02:45 PM
Jun 2014

I trust her more than that article...


And HER paycheck has nothing to do with that....

She also rails against the Antivaxx crowd too

 

GliderGuider

(21,088 posts)
5. People "in the industry" with PhDs miscalculate risks all the time.
Wed Jun 11, 2014, 02:48 PM
Jun 2014

You may trust her. I would prefer to keep DNA fragments of GM food out of my bloodstream until we have done a little (no, a LOT) more unbiased research. Precautionary Principle and all that.

 

VanillaRhapsody

(21,115 posts)
6. there is no way to do that....if you eat it...it enters your body....
Wed Jun 11, 2014, 02:49 PM
Jun 2014

If you eat petty much any crop at all it has over time been genetically modified....even without the technology we have today....just saying.

Drew Richards

(1,558 posts)
29. UMmm NOT with what they are genetically modifying with...IE PIG Genes Frog Genes ...give me a break.
Wed Jun 11, 2014, 04:37 PM
Jun 2014

Big damn difference between natural selection and human organic hybridization of plants vs Genetic modification introducing ANIMAL proteins and RNA into a plant to make it...more drought resistant or higher abundant...

Sorry...

Even if this article is lame, biased and unscientifically authenticated the premise is still there...

We should not be gambling with what nature never intended or qualified in nature IE adding animal proteins to plants for genetic modification for consumption...

MattBaggins

(7,897 posts)
10. Should I instead trust
Wed Jun 11, 2014, 03:15 PM
Jun 2014

Random internet articles that take a simple biological process and blow it way out of proportion?

REP

(21,691 posts)
37. Well yes. I know my ass from third base. Ho not so much.
Wed Jun 11, 2014, 08:18 PM
Jun 2014

But hey, follow whomever you want. It neither breaks my leg nor picks my pocket; just don't try to make me or others come along.

 

AverageJoe90

(10,745 posts)
44. Scientists themselves can be falliable, this is true.
Thu Jun 12, 2014, 07:47 PM
Jun 2014

For example, with climate change, you still have those who deny that it even exists, or claim that it will only be good(yeah, not really) for the planet....and on the other extreme, you've got guys like David Wasdell or Guy McPherson, or John Greer, for example, who toot the horn of "Inevitable Human Permanent Decline/Extinction" every so often, based on nothing but rhetoric, and the occasional research, either poorly done hackwork or badly misintrepreted pieces of legitimate research.

You've also got a fair number of scientists who insist that consciousness is literally nothing more than brain fireworks, or that all NDEs are nothing more than fancy-pants illusions created by a malfunctioning brain, or that humans are actual apes(instead of being evolutionary "cousins" to apes, like raccoons are to bears, but perhaps a little closer), etc.

BTW, I'm not exactly a big fan of GMOs myself. I don't buy into any of the gloom-and-doom scenarios that are sometimes thrown around the place, but there are indeed legitimate concerns about their use, or misuse.

REP

(21,691 posts)
19. Not so much
Wed Jun 11, 2014, 03:31 PM
Jun 2014
Ho is the director of the Institute of Science in Society (ISIS), an interest group that campaigns against what it sees as unethical uses of biotechnology.[6] The group published about climate change, GMOs, homeopathy, traditional Chinese medicine, and water memory.

In reviewing the organisation, David Colquhoun accused the ISIS of promoting pseudoscience and specifically criticised Ho's understanding of homeopathy.[7]


Ho has expressed concerns about the spread of altered genes through horizontal gene transfer and that the experimental alteration of genetic structures may be out of control. One of her concerns is that the antibiotic resistant gene that was isolated from bacteria and used in some GM crops might cross back from plants by horizontal gene transfer to different species of bacteria, because "If this happened it would leave us unable to treat major illnesses like meningitis and E coli."[8] Her views were published in an opinion article based on a review of others' research.[9] The arguments and conclusions of this article were heavily criticized by prominent plant scientists,[10] and the claims of the article criticized in detail in a response that was published in the same journal.[11] A review on the topic published in 2008 in the Annual Review of Plant Biology stated that "These speculations have been extensively rebutted by the scientific community".[12]

Ho, together with Joe Cummins of the University of Western Ontario, has argued that a sterility gene engineered into a crop could be transferred to other crops or wild relatives and that "This could severely compromise the agronomic performance of conventional crops and cause wild relatives to go extinct". They argued that this process could also produce genetic instabilities, which might be "leading to catastrophic breakdown", and stated that there are no data to assure that this has not happened or cannot happen.[13] This concern contrasts with the reason why these sterile plants were developed, which was to prevent the transfer of genes to the environment by preventing any plants that are bred with or that receive these genes from reproducing.[14] Indeed, any gene that caused sterility when transferred to a new species would be eliminated by natural selection and could not spread.[15]

Ho has also argued that bacteria could acquire the bacterial gene barnase from transgenic plants. This gene kills any cell that expresses it and lacks barstar, the specific inhibitor of barnase activity. In an article entitled Chronicle of An Ecological Disaster Foretold, which was published in an ISIS newsletter, Ho speculated that if a bacterium acquired the barnase gene and survived, this could make the bacteria a more dangerous pathogen.[16]


http://en.m.wikipedia.org/wiki/Mae-Wan_Ho#Institute_of_Science_in_Society

G_j

(40,366 posts)
27. Upon looking further
Wed Jun 11, 2014, 04:20 PM
Jun 2014

Last edited Thu Jun 12, 2014, 03:15 AM - Edit history (1)

ISIS does seem to have a certain agenda. However, a pharmacologist who is particularly critical of alternative medicine?.. I'd look somewhere else..

REP

(21,691 posts)
36. He seems especially dismissive of expensive water, or homeopathy as it is often called
Wed Jun 11, 2014, 08:16 PM
Jun 2014

He's a researcher specializing in ion gap, which is somewhat interesting to those trying to treat certain intractable diseases. But carry on.

jeff47

(26,549 posts)
9. Your post demonstrates the need for better eductation
Wed Jun 11, 2014, 03:11 PM
Jun 2014

DNA in your blood can't change DNA in your cells. Your DNA is in the nucleus of your cells. It's only there that your DNA can have an effect.

In fact, viruses have to work extremely hard to get to the point where they can infect your cells, because of that barrier. People who make genetically modified food also have to work very hard to get their modified genes to actually do something. You can't just dump some DNA on a plant and have it change the plant.

But let's take a look at the article:

Previous animal feeding studies have demonstrated that a minor proportion of fragmented dietary DNA may resist digestion, but the degradation of long chains of DNA and the possible uptake and transport into the bloodstream are not at all understood. Circulating cell free DNA (cfDNA) in the human bloodstream, first described in 1948, are mostly double-stranded molecules with a wide range of fragment sizes from 180 - 21 k bp.

So this article is covering a shocking new discovery....from 1948.

But it does to an excellent job of salesmanship!
 

GliderGuider

(21,088 posts)
12. The conclusion of the report says this:
Wed Jun 11, 2014, 03:16 PM
Jun 2014
Nucleic acids (both DNA and RNA) from food can resist digestion in the human gut and enter the circulatory system, with the potential of being taken up by cells to influence gene expression and/or become incorporated into the cell’s genome. This underscores the hazards of GM and other unknown nucleic acids introduced into the human food chain by GMOs.

I don't know how great the potential for uptake is, but maybe it's not me that needs edumacating...

pnwmom

(108,953 posts)
15. Monsanto has to sell its GMO's so they can sell their herbicides
Wed Jun 11, 2014, 03:19 PM
Jun 2014

in their wonderful circle of profit.

jeff47

(26,549 posts)
18. And that conclusion is talking about what we discovered in 1948.
Wed Jun 11, 2014, 03:29 PM
Jun 2014
I don't know how great the potential for uptake is, but maybe it's not me that needs edumacating

No, it's still you.

cfDNA has not been shown to affect anything. We've known about it for 66 years. If it had an effect, don'tcha think we'd have found it by now?

But you can get people excited, and receive more funding, if you throw out GMOs as a boogeyman. And when you have not-really-a-journal sites like ISIS willing to publish whatever you throw at them, it's even better.

All you need is a crowd of people who don't understand the basics of biology and DNA, and then pretend GMOs are magical beasts created in sinister Transylvanian labs by magical processes. And pretend that the magic leaks all over anything that the GMOs touch.
 

GliderGuider

(21,088 posts)
41. My degree is not in molecular biology. However,
Thu Jun 12, 2014, 10:04 AM
Jun 2014

Since yours apparently is, perhaps you could comment on this paper from Nature (from 2011, not 1948): Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

The reason I ask is that is seems to provide evidence of just the kind of cross-kingdom genetic effects I'm interested in. So far, the only counter to it I've found is this: Plant RNA Paper Questioned. As you can see, this is not a peer-reviewed study, but simply an article exploring a an opinion controversy of the sort that always surrounds new scientific findings.

If you could debunk the original research (with peer-reviewed references of course) I'd be ever so grateful.

jeff47

(26,549 posts)
43. Not genes being expressed.
Thu Jun 12, 2014, 07:36 PM
Jun 2014
Since yours apparently is, perhaps you could comment on this paper from Nature (from 2011, not 1948): Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

That's signalling, not genes being expressed. The concern from ISIS is gene expression.

This paper is about as exciting and novel as both creatures using the same amino acids. They may use the same signalling mechanism....which we haven't proven is a signalling mechanism, btw.

The reason I ask is that is seems to provide evidence of just the kind of cross-kingdom genetic effects I'm interested in.

Keyword searches are not terribly good at proving things.
 

GliderGuider

(21,088 posts)
40. A couple of comments
Thu Jun 12, 2014, 09:42 AM
Jun 2014

"cfDNA has not been shown to affect anything" is not the same as "cfDNA has been shown not to affect anything". Your formulation leaves a lot of room for further research. That's a good thing, right?

"But you can get people excited, and receive more funding, if you throw out GMOs as a boogeyman." You seem to be saying that receiving more funding for molecular biology research is a bad thing. But you can't actually mean that, can you?

I get the distinct impression that you're trying to shut down the discussion. But that can't be true either, can it?

jeff47

(26,549 posts)
42. :eyes:
Thu Jun 12, 2014, 07:34 PM
Jun 2014
"cfDNA has not been shown to affect anything" is not the same as "cfDNA has been shown not to affect anything".

There's a very long laundry list of things we have not shown to happen. Burn a piece of paper. We have not shown it's impossible for the CO2 to re-form into paper. You gonna expect it to happen? For how long?

Additionally, no one has found any situation where eukaryotes (non-bacteria) can take up foreign DNA on their own. It has to be injected by something else, like a virus or a genetic engineer. That's step one in cfDNA getting expressed and it has never been shown to happen.

You seem to be saying that receiving more funding for molecular biology research is a bad thing.

It is when you're "research" is about lying.

Just like additional funding for anti-vaxxers is a bad thing. Or additional funding for climate change deniers.

I get the distinct impression that you're trying to shut down the discussion.

No, I'm attempting to point out that there are people shoveling bullshit that they pretend is science in order to defraud the public.
 

VanillaRhapsody

(21,115 posts)
16. basically what my Sister in Law explained to me when I showed her this article a couple weeks back
Wed Jun 11, 2014, 03:20 PM
Jun 2014

That this is hyperbolic nonsense...

MineralMan

(146,248 posts)
14. Although it might look like it, that
Wed Jun 11, 2014, 03:19 PM
Jun 2014

is not a scientific article from peer-reviewed journal. Instead, it is just an article published on an advocacy website. While it sort of masquerades as a scientific article, it is not, and ISIS is not a scientific journal at all.

In fact, ISIS is an advocacy organization and nothing published there should be accepted as fact without further review.

Very clever, ISIS, following typical journal article formatting, but nobody should be fooled into thinking that's what this is.

REP

(21,691 posts)
21. About the Institute and Ho:
Wed Jun 11, 2014, 03:38 PM
Jun 2014
Ho is the director of the Institute of Science in Society (ISIS), an interest group that campaigns against what it sees as unethical uses of biotechnology.[6] The group published about climate change, GMOs, homeopathy, traditional Chinese medicine, and water memory.

In reviewing the organisation, David Colquhoun accused the ISIS of promoting pseudoscience and specifically criticised Ho's understanding of homeopathy.[7]


Ho has expressed concerns about the spread of altered genes through horizontal gene transfer and that the experimental alteration of genetic structures may be out of control. One of her concerns is that the antibiotic resistant gene that was isolated from bacteria and used in some GM crops might cross back from plants by horizontal gene transfer to different species of bacteria, because "If this happened it would leave us unable to treat major illnesses like meningitis and E coli."[8] Her views were published in an opinion article based on a review of others' research.[9] The arguments and conclusions of this article were heavily criticized by prominent plant scientists,[10] and the claims of the article criticized in detail in a response that was published in the same journal.[11] A review on the topic published in 2008 in the Annual Review of Plant Biology stated that "These speculations have been extensively rebutted by the scientific community".[12]

Ho, together with Joe Cummins of the University of Western Ontario, has argued that a sterility gene engineered into a crop could be transferred to other crops or wild relatives and that "This could severely compromise the agronomic performance of conventional crops and cause wild relatives to go extinct". They argued that this process could also produce genetic instabilities, which might be "leading to catastrophic breakdown", and stated that there are no data to assure that this has not happened or cannot happen.[13] This concern contrasts with the reason why these sterile plants were developed, which was to prevent the transfer of genes to the environment by preventing any plants that are bred with or that receive these genes from reproducing.[14] Indeed, any gene that caused sterility when transferred to a new species would be eliminated by natural selection and could not spread.[15]

Ho has also argued that bacteria could acquire the bacterial gene barnase from transgenic plants. This gene kills any cell that expresses it and lacks barstar, the specific inhibitor of barnase activity. In an article entitled Chronicle of An Ecological Disaster Foretold, which was published in an ISIS newsletter, Ho speculated that if a bacterium acquired the barnase gene and survived, this could make the bacteria a more dangerous pathogen.[16]


http://en.m.wikipedia.org/wiki/Mae-Wan_Ho#Institute_of_Science_in_Society

yawnmaster

(2,812 posts)
28. Not confirmed! Hyperbole and mis-information. Non-scientific...
Wed Jun 11, 2014, 04:33 PM
Jun 2014

As others have stated in this thread, this is not research and is not from a peer reviewed journal and really doesn't given any new information. It just puts information together in a way that is propaganda and hyperbole.

DNA entering a cell, much less eventually getting expressed as a protein is by no means trivial and is near (if not) impossible!

And so easily testable too, with a human cell culture with DNA in the media.
It doesn't happen.
The cell has numerous innate mechanisms preventing this...serious mechanisms.
For instance, how does the DNA get into the nucleus to be transcribed into RNA??
how does it get past the cell membrane?? and these two are mechanical barriers.
There are numerous active barriers.
You can argue GMO and I am all for rational argument, but this is not one of them.

 

DeSwiss

(27,137 posts)
32. K&R!
Wed Jun 11, 2014, 04:59 PM
Jun 2014


[font size=3]Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA[/font]

Abstract

Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 (LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

CELL RESEARCH: Full Study


On edit: ScienceDaily: ''Plant miRNAs could enter host blood and tissues via food intake, study suggests''

 

GliderGuider

(21,088 posts)
34. Hmmm. Well would you look at that.
Wed Jun 11, 2014, 05:04 PM
Jun 2014

And from Nature no less. I wonder if it was peer reviewed? </sarcasm>

 

DeSwiss

(27,137 posts)
35. Yeah.....
Wed Jun 11, 2014, 05:49 PM
Jun 2014

...the GMOers here won't touch it ever since I posted it earlier. They. Have. Nothing.

It destroys all their BS and obfuscation attempts.

- Truth does that to those who live in The Dark......

We don't have to live this way......

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