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Boosting Biogasoline Production in Microbes
http://newscenter.lbl.gov/2014/10/27/boosting-biogasoline-production-in-microbes/[font face=Serif][font size=5]Boosting Biogasoline Production in Microbes[/font]
[font size=4]Joint BioEnergy Institute Researchers Combine Systems Biology with Genetic Engineering to Improve Production of Isopentenol in E.Coli[/font]
News Release Lynn Yarris (510) 486-5375 • October 27, 2014
[font size=3]In the on-going effort to develop advanced biofuels as a clean, green and sustainable source of liquid transportation fuels, researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have identified microbial genes that can improve both the tolerance and the production of biogasoline in engineered strains of Escherichia coli.
Aindrila Mukhopadhyay, a chemist who directs the host engineering program for JBEI’s Fuels Synthesis Division, led a study in which transcriptomic data and a synthetic metabolic pathway were used to identify several genes that not only improve tolerance but also production of isopentenol in E.coli. Isopentenol is a five-carbon alcohol that is a highly promising candidate for biogasoline, but, like other short-chained alcohols, is toxic to E.coli at commercial levels of fuel production.
“Our study demonstrates that microbial tolerance engineering using transcriptomics data can be used to identify target genes that improve fuel production,” says Mukhopadhyay, who also holds an appointment with the Lawrence Berkeley National Laboratory (Berkeley Lab)’s Physical Biosciences Division. “Our targets include a regulator for amino acid biosynthesis, and an ABC transporter protein, the first native transporter that improves tolerance to a short-chain alcohol.”
…
“MetR, the methionine biosynthesis regulator, improved the titer for isopentenol production by 55-percent,” Mukhopadhyay says. “MdlB, the ABC transporter, facilitated a 12-percent improvement in isopentenol production.”
…[/font][/font]
[font size=4]Joint BioEnergy Institute Researchers Combine Systems Biology with Genetic Engineering to Improve Production of Isopentenol in E.Coli[/font]
News Release Lynn Yarris (510) 486-5375 • October 27, 2014
[font size=3]In the on-going effort to develop advanced biofuels as a clean, green and sustainable source of liquid transportation fuels, researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have identified microbial genes that can improve both the tolerance and the production of biogasoline in engineered strains of Escherichia coli.
Aindrila Mukhopadhyay, a chemist who directs the host engineering program for JBEI’s Fuels Synthesis Division, led a study in which transcriptomic data and a synthetic metabolic pathway were used to identify several genes that not only improve tolerance but also production of isopentenol in E.coli. Isopentenol is a five-carbon alcohol that is a highly promising candidate for biogasoline, but, like other short-chained alcohols, is toxic to E.coli at commercial levels of fuel production.
“Our study demonstrates that microbial tolerance engineering using transcriptomics data can be used to identify target genes that improve fuel production,” says Mukhopadhyay, who also holds an appointment with the Lawrence Berkeley National Laboratory (Berkeley Lab)’s Physical Biosciences Division. “Our targets include a regulator for amino acid biosynthesis, and an ABC transporter protein, the first native transporter that improves tolerance to a short-chain alcohol.”
…
“MetR, the methionine biosynthesis regulator, improved the titer for isopentenol production by 55-percent,” Mukhopadhyay says. “MdlB, the ABC transporter, facilitated a 12-percent improvement in isopentenol production.”
…[/font][/font]
